| Objective: |
To secure employment in a progressive and challenging
research position where my strengths in chemistry, biochemistry, and molecular
biology will be utilized effectively. |
| |
|
| Education: |
Ph. D. in Nutrition Sciences, degree expected
June 1999 |
| |
University of California, Davis, California |
| |
M. S. in Food Science and Human Nutrition,
January 1994 |
| |
University of Florida, Gainesville, Florida |
| |
B. S. in Chemistry, May 1991 |
| |
Colorado State University, Fort Collins, Colorado |
| |
Professional Certification by the American Chemical
Society |
| |
|
| Skills: |
|
| Chemistry |
LPLC, reverse phase HPLC, FPLC, protein purification,
mass spectrometry, kinetic modeling, radioactive techniques, UV-VIS, FTIR,
NMR, small molecule synthesis. |
| |
|
| Molecular |
DNA enzyme restriction digestion, DNA ligation,
Southern analysis, Western blots, DNA plasmid mini, midi, and maxi purification,
elecrophoresis, microbiological techniques, electroblotting, electrocompetent
cells. |
| |
|
| Animals |
animal breeding, animal care and husbandry,
oral gavage, intraperitoneal injections, animal surgery, tissue extraction,
preparation of chemically defined diet |
| |
|
| Biological |
cell culture techniques, isolation of intact
mitochondria from fibroblasts and liver cells, isolation of functional
mitochondria from mouse liver, mitochondrial respiration measurements,
electron microscopy, photographic development, thiobarbituric acid reacting
substances, glutamine synthetase, protein carbonyls, 8-oxo-2-deoxyguanosine,
vitamin C, PQQ redox cycling, glucose dehydrogenase, cytochrome oxidase
antibody, glucose oxidase, Bradford protein, Lowrey protein, alanine amino
transferase, glutathione. |
| |
|
| Computer |
Proficient in the use of both PC and Macintosh
computers. Software programs include Excel, Word, Power Point, WordPerfect,
Cricket Graph, Kaleidagraph, DeltaGraph, Statview, JumpIn Statistics, ChemDraw,
Netscape, and Internet Explorer. |
| |
|
| Research Experience: |
University of California, Davis: January
1994 to Present
(Advisor: Dr. Robert B Rucker) |
| |
-
Absorption and tissue distribution of 14C-pyrroloquinoline quinone
(PQQ) in mice.
|
| |
-
Synthesis of 14C-PQQ in E coli with a plasmid from K.
Pneumoniae.
|
| |
-
In vivo antioxidant properties of PQQ and related derivatives in
mouse liver.
|
| |
-
Uptake and localization of 3H-PQQ in human fibroblasts and liver
cells.
|
| |
-
Cocoa supplementation improved growth and reproductive performance in mice.
|
| |
-
Determination of the importance of PQQ for liver mitochondrial function.
|
| |
-
The interactions of pyrroloquinoline quinone and diphenylene iodonium in
mice.
|
| |
|
| |
University of Florida: July 1991 to December
1993:
(Advisor: Dr. Jesse F. Gregory III) |
| |
-
Design of a kinetic modeling and bioavailability protocol in human subjects.
|
| |
-
Synthesis of deuterium labeled folate compounds used in human study.
|
| |
-
Analysis and purification of folate samples using reverse phase HPLC.
|
| |
-
Determination of isotopic enrichment of urinary folate using GC/MS.
|
| |
-
Utilization of SAAM kinetic modeling program to model kinetic data.
|
| |
|
| |
Colorado State University: June 1987 to June
1991:
(Advisor: Dr. Debbie Crans) |
| |
-
Independent research in vanadate chemistry.
|
| |
-
Enzyme kinetics of glucose-6-phosphate dehydrogenase (G6PDH).
|
| |
-
Synthesis of inorganic vanadate inhibitors of G6PDH.
|
| |
|
| Teaching Experience: |
University of California: Davis, California
Nutritional Assessment (NUT112)
Instructor of a laboratory course for two years. The course
focus was on methods of nutritional assessment. Topics covered included
dietary, biochemical, anthropometric, and hematological techniques to analyze
nutritional status in human subjects.
Current Topics and Controversies in Nutrition (NUT11)
Instructor of a course on how to read and understand nutrition research
articles. Students learned how to interpret scientific data, how to give
oral and written reports, and how to write term papers on research articles.
Principles of Nutrition (NUT110)
Teaching assistant for a nutrition course with a metabolism emphasis.
Responsibilities included grading exams and holding office hours to answer
students’ questions.
Food Service Management (FSM123)
Teaching assistant for a management theories course. Responsibilities
included grading exams, term papers, and oral presentations.
University of Florida: Gainesville, Florida
Food Chemistry (FOS4311)
Taught a series of lectures on food proteins, amino acid in foods,
food enzymes, enzyme kinetics, and enzyme use in food processing techniques.
Fundamentals of Human Nutrition (HUN2201)
Taught a series of lectures on the properties, functions, requirements,
interrelationships and metabolism of nutrients.
Nutrition and Metabolism (HUN3221)
Guest lecturer for a nutrition course with a clinical emphasis. Instructed
students on the biochemical causes and clinical treatments for hypertension.
Colorado State University: Fort Collins, Colorado
Organic Chemistry (CHE301)
Responsibilities included grading written organic chemistry
exams and proctoring exams.
|
| |
|
| Publication: |
-
Stites, T. E., Bailey, L. B., Scott, K. C., Toth, J. P., Fisher,
W. P., & Gregory, J. F. (1997) Kinetic modeling of folate metabolism
through use of chronic administration of deuterium-labeled folic acid in
men. Am J Clin Nutr. 65(1): 53-60.
|
| |
-
Rucker, R. B., & Stites, T. (1994) New perspectives on function
of vitamins. Nutrition. 10(6): 507-13.
|
| |
|
| Abstracts: |
-
Stites, T.E., Ly, J.Q., Cui, C., and Rucker, R.B. (1999) The antioxidant
properties of pyrroloquinoline quinone (PQQ) and related derivatives in
mice. FASEB Journal of Experimental Biology Abstract.
|
| |
-
Stites, T.E., and Rucker, R.B. (1999) Cocoa supplementation improved
growth and reproductive performance in mice fed chemically defined PQQ
deficient diets. FASEB Journal of Experimental Biology Abstract.
|
| |
-
Cui, C., Stites, T.E., and Rucker, R.B. (1999) Influence of dietary
vanadium on copper accumulation and lysyl oxidase activity in rat tendon.
FASEB
Journal of Experimental Biology Abstract.
|
| |
-
Sites, T.E., Mitchell, A., and Rucker, R.B. (1998) Synthesis of
14-C-pyrroloquinoline quinone (PQQ) in E-coli organisms using 14-C-tyrosine
and PQQ synthesis genes from K. Pneumoniae. FASEB Journal of
Experimental Biology Abstract # 1444.
|
| |
-
Sites, T. E., Mah, J., Flückiger, R., Paz, M., Gallop, P.,
and Rucker, R. (1996) Dietary deficiency of pyrroloquinoline quinone (PQQ)
alters mitochondrial function in young mice. FASEB Journal of Experimental
Biology Abstract #4622.
|
| |
-
Sites, T., Mitchell, M., Rose, M., Rucker, B., & Rucker, R.
(1995) The interactions of pyrroloquinoline quinone and diphenylene iodonium
in young mice. FASEB Journal of Experimental Biology Abstract # 5731.
|
| |
-
Sites, T., Gregory III J., (1994) Kinetic modeling of isotopically
administered folate in human subjects. FASEB Journal of Experimental
Biology Abstract # 4082.
|
| |
|
| Awards: |
1995-96, 1996-97: Jastro Shields Research Fellowship
University of California, Davis. |
| |
1994: Fellowship Award: University of California,
Davis. |
| |
1991: Gustafson Award: Undergraduate Research
award at Colorado State University for the best undergraduate researcher
in the Department of Chemistry. |
| |
1991: American Chemical Society Undergraduate
Research Conference Invitation |
| |
|
| Professional Affiliations
|
American Chemical Society
American Society of Nutritional Sciences
American Society of Clinical Nutrition
Institute of Food Technology |
| |
|
| References: |
Available upon request |